Intended Audience: Pathologists, Residents, Clinical Scientists, Pathologist Assistants
Upon completion of this activity, you will be able to:
- describe the similarities and differences in the clinical evaluation of T-cell clonality in hematolymphoid specimens by two main currently used molecular...
Back to The Value of T-Cell Receptor Gamma (TRG) Clonality Evaluation by Next-Generation Sequencing in Clinical Hematolymphoid Tissues: A Descriptive Study of 41 Cases From a Single Institution
Intended Audience: Pathologists, Residents, Clinical Scientists, Pathologist Assistants
Upon completion of this activity, you will be able to:
- describe the similarities and differences in the clinical evaluation of T-cell clonality in hematolymphoid specimens by two main currently used molecular methods (fragment length polymerase chain reaction assays with capillary electrophoretic detection of rearranged Vgamma-Jgamma DNA sequences compared with next-generation sequencing [NGS]–based evaluation of rearranged Vgamma-Jgamma DNA sequences, including advantages for clonality analysis by NGS).
- apply that knowledge for definitive evaluation of T-cell clonality by NGS in the most commonly submitted clinical specimens (ie, in peripheral blood, fresh [nonfixed] bone marrow aspirates, and formalin-fixed, paraffin-embedded tissues), including for evaluation of preanalytic technical aspects critical for successful clonality evaluation by NGS.
- analyze data derived from T-cell receptor gamma NGS assays for clonality in clinical cases to rule out or rule in the presence of a T-cell clone (including, if present, a quantitative estimate of the minimum percentages of the clonal population within all T cells and the nature of the clonal population as biallelic or monoallelic or possibly involving multiple Vgamma-Jgamma regions in any given case).