Release Date: Jul 2019
CC: PC, MK
Stacey Robinson, MS, MLS(ASCP)SH, SCYM
Supervisor of Clinical Microscopy, Walter Reed NMMC, Bethesda, MD
After completing this course, participants will be able to:
Who should attend? Residents, Bench Supervisors, Bench Technologists & Technicians, Students
Flow cytometry has become commonplace in hospitals, reference laboratories, and in some instances even in smaller hospitals and clinics. It is the principle behind most of the automated cell counters we use today. For a new leukemia or lymphoma case, it is often the quickest way to gain a large amount of information and potentially get treatment started early. Now, with the arrival of 8- and 10-color flow in clinical laboratories, even more information can be gathered from even fewer cells. It is a relatively simple technique, but the quality control (QC) requirements can seem relatively complex.
This course will review some of the possible methods for meeting the QC requirements in a clinical flow cytometry laboratory. Topics will include analyzer QC using setup beads, standardizing the output between cytometers in the same laboratory, compensation, antibody QC, and kit QC and validation.
This is a basic overview of quality control in clinical flow cytometry for the technologist who is new to the flow cytometry laboratory. It is also intended to be useful for the supervisor who is not trained in flow cytometry or for the technologist establishing a QC plan for a new flow cytometry laboratory.