LabQ Histology Exercise 3: Histology: Utility of Paraffin Immunofluorescence in the Evaluation of Kidney Biopsy

This is an online activity in Clinical Chemistry that offers 2.0 continuing education (CMLE) credit that can be used to fulfill CMP requirements.

Selvaraj Muthusamy MBBS, PhD 

Virginia Commonwealth University, 

Richmond, Virginia 

Primary Audience:

Phlebotomists, Laboratory Technologists, Laboratory Directors

Secondary Audience:

Pathologists, Clinical Scientists (Includes all sub-specialty areas), Students, Pathologist Assistants, Residents, and Students

Upon completion of this activity, you will be able to: 

·        summarize the role of light microscopy, immunofluorescence, and electron microscopy in the evaluation of renal biopsy; 

·        discuss the role of additional histochemical and immunohistochemical stains in the interpretation of kidney biopsy; 

·        explain the importance of correlating findings of light microscopy, immunofluorescence, and electron microscopy to avoid diagnostic pitfalls. 

·        recognize possible signs of hidden epitopes when evaluating immunofluorescence findings; and 

·        discuss the technique and limitations of paraffin immunofluorescence.