LabQ Clinical Exercise 6: Clinical Immunology: The Importance of Lymphocyte Gate Purity in T-, B-, and NK-Cell Immunophenotyping

Kim Shumate, ASCPCM 

Medical Technologist and Hematopathology Laboratory 

Children’s Hospital Colorado 

Aurora, Colorado 

Vijaya Knight, MD, PhD, (D)ABMLI  

Department of Pediatrics  

Section of Allergy & Immunology   

University of Colorado School of Medicine and Children’s Hospital Colorado      

Aurora, Colorado

Primary Audience:

Phlebotomists, Laboratory Technologists, Laboratory Directors

Secondary Audience:

Pathologists, Clinical Scientists (Includes all sub-specialty areas), Students, Pathologist Assistants, Residents, and Students

Upon completion of this activity, you will be able to: 

·        describe the cell subsets evaluated through lymphocyte subset immunophenotyping; 

·        describe different gating strategies used for peripheral blood lymphocyte phenotyping; 

·        discuss peripheral blood lymphocyte markers; and 

·        explain the impact of monocyte contamination on lymphocyte subset immunophenotyping.