Case Reports Clinical Chemistry 2024 Exercise 5: Mouse in the Mix: Human Anti-Mouse Antibody Interference in Clinical Assays

Authors

Catherine Omosule, PhD, DABCC

Medical And Scientific Research Centre

University Of Ghana Medical Centre

Patrick Seidu, MBCHB

Department of Internal Medicine

Korle-Bu Teaching Hospital

Accra, Ghana

Primary Audience: Pathologists and Clinical Scientists
Secondary Audience: Residents, Laboratory Technologist/Scientist (Includes all sub-specialty areas), Students, Pathologist Assistants,  Laboratory Directors and Educators

Upon completion of this activity, you will be able to:

• explain the etiology and development of heterophile antibodies and human anti-mouse antibodies (HAMA);
• recognize clinical situations that merit evaluation of HAMA in patients;
• formulate strategies to test for and overcome heterophile antibody interference on immunoassays; and
• describe the analytic challenges posed by HAMA interference in immunoassays, including its potential impact on diagnostic accuracy and patient care.